NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM104726 Query DataSets for GSM104726
Status Public on Jul 14, 2006
Title 48h_IVS Sham tissue, biological rep2, Cv2
Sample type RNA
 
Source name C57/BL6 heart tissue
Organism Mus musculus
Characteristics Weight of mouse: 25-30g
Age: 8-10 wk old
Treatment protocol Permanent occlusion of the Left Anterior Descending coronary artery was performed on adult male (8 to 10 wk old) C57BL/6J mice. To exclude surgical effects on gene expression, sham surgeries were conducted by passing suture under the artery without ligation. Animals were sacrificed at time t=0 (naïve control) and at each time point post-AMI (0.25, 1, 4, 12, 24 and 48 h)
At each time point 4-10 animals were sacrificed and hearts harvested, regionally dissected and immediately frozen in liquid N2.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual frozen tissue samples using an SDS/phenol isolation protocol after which equal weights of RNA from 4-10 mice were pooled for each dissected tissue type.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from different regions of the mouse heart
Data processing The data were analyzed with Robust Multi-array Averaging (RMA; www.bioconductor.org) and global scaling (across 192 chips)as normalization method.
 
Submission date Apr 10, 2006
Last update date Jul 11, 2006
Contact name Mark Stayton
E-mail(s) stayton@uwyo.edu
Phone 307 766-4025
Fax 307 766-5098
Organization name University of Wyoming
Department Molecular Biology
Street address Dept. 3944, 1000 E. University Ave.
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL83
Series (1)
GSE4648 Earliest Changes in the Left Ventricular Transcriptome Post-Myocardial Infarction

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
128520_at 709.9811936
128523_at 9.240462099
128529_at 19.85810428
128550_at 73.71340007
128554_at 33.88786871
128577_s_at 18.17826998
128578_r_at 41.3595906
128579_r_at 25.61018909
128580_at 59.12773058
128582_at 13.98099371
128583_at 14.05970092
128590_at 38.59360751
128624_at 10.95647642
128643_r_at 394.3721872
128644_at 212.1417846
128645_at 53.86163055
128646_at 19.36562288
128647_at 43.29252374
128651_at 102.4375611
128652_at 20.0485362

Total number of rows: 11934

Table truncated, full table size 264 Kbytes.




Supplementary file Size Download File type/resource
GSM104726.CEL.gz 2.7 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap