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Sample GSM10482 Query DataSets for GSM10482
Status Public on Mar 31, 2005
Title lec1-1 Mature Green Seed 2
Sample type RNA
 
Source name Genotype: Ws-0, lec1-1; Stage: Mature Green Seed; Biological Replica: 2 of 2
Organism Arabidopsis thaliana
Extracted molecule total RNA
 
Description Growth Condition: Plants were grown in a Conviron chamber under continuous light with fluorescent lamps at 20oC and 50% - 70% relative humidity. Experiments were performed as described (Le et. al., 2003, PNAS).


Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2003, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 5.0 (MAS 5.0) with a global scaling intensity set at 500.
Keywords = Arabidopsis
Keywords = seed development
 
Submission date Sep 25, 2003
Last update date Aug 28, 2018
Contact name Bob Goldberg
E-mail(s) bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL198
Series (1)
GSE1051 Seed development in LEAFY COTYLEDON1 mutants
Relations
Reanalyzed by GSE119083

Data table header descriptions
ID_REF
VALUE This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
Detection p-value A p-value indicating the significance of the Detection call

Data table
ID_REF VALUE ABS_CALL Detection p-value
AFFX-BioB-5_at 966.8 P 0.00011
AFFX-BioB-M_at 1714.1 P 0.000044
AFFX-BioB-3_at 1476.3 P 0.000044
AFFX-BioC-5_at 2546.6 P 0.000052
AFFX-BioC-3_at 1345.8 P 0.00007
AFFX-BioDn-5_at 2519.8 P 0.000044
AFFX-BioDn-3_at 9920.4 P 0.000044
AFFX-CreX-5_at 21873.6 P 0.000044
AFFX-CreX-3_at 26721.8 P 0.000044
AFFX-DapX-5_at 3 A 0.941556
AFFX-DapX-M_at 44.2 A 0.250796
AFFX-DapX-3_at 3.8 A 0.971543
AFFX-LysX-5_at 4.8 A 0.724854
AFFX-LysX-M_at 4.6 A 0.937071
AFFX-LysX-3_at 5.7 A 0.631562
AFFX-PheX-5_at 8.8 A 0.876428
AFFX-PheX-M_at 5.3 A 0.772364
AFFX-PheX-3_at 15.4 A 0.9273
AFFX-ThrX-5_at 25 A 0.574038
AFFX-ThrX-M_at 7.1 A 0.58862

Total number of rows: 22810

Table truncated, full table size 588 Kbytes.




Supplementary file Size Download File type/resource
GSM10482.CEL.gz 3.2 Mb (ftp)(http) CEL

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