|
| Status |
Public on Dec 05, 2012 |
| Title |
MGC-803_cells_synbindin_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MGC-803 cells infected with synbindin adenoviruses
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MGC-803
cell type: gastric cancer cell line
treatment: synbindin adenoviruses
|
| Treatment protocol |
MGC-803 cells were treated with adenoviruses carrying synbindin or control viruses carrying GFP.
|
| Growth protocol |
MGC-803 cells were maintained at 37 °C in an atmosphere of 5% CO2 in RPMI-1640 medium (Invitrogen) supplemented with 10% fetal bovine serum, penicillin and streptomycin (Invitrogen) in 25-ml culture flasks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Forty-eight hours after treatment, total RNA from each sample was isolated and quantified. Integrity and concentration of RNA were assessed after RNA extraction and prior to sample labeling.
|
| Label |
Cy3
|
| Label protocol |
The Agilent Quick Amp Labeling Kit was used for sample labeling. Briefly, total RNA from each sample was amplified and transcribed into fluorescent cRNA using Agilent's Quick Amp Labeling protocol (version 5.7, Agilent Technologies).
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Whole Human Genome Oligo Microarray (4 x 44K, Agilent Technologies).
|
| Scan protocol |
After washing, the arrays were scanned by the Agilent Scanner G2505C.
|
| Description |
synbindin_3
Gene expression after synbindin adenovirus infection.
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies).
|
| |
|
| Submission date |
Dec 04, 2012 |
| Last update date |
Dec 05, 2012 |
| Contact name |
Jie Xu |
| E-mail(s) |
xujieletter@gmail.com
|
| Organization name |
Shanghai Institute for Digestive Diseases
|
| Department |
Shanghai Renji Hospital
|
| Lab |
State Key Laboratory for Oncogenes and Related Genes
|
| Street address |
Shandong Rd 145
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL13497 |
| Series (1) |
| GSE42736 |
Effect of synbindin on the gene expression profile of gastric cancer cells |
|
