|
Status |
Public on Dec 13, 2012 |
Title |
Control rep 2 |
Sample type |
RNA |
|
|
Source name |
Adult ovary from room air exposed mouse
|
Organism |
Mus musculus |
Characteristics |
tissue: Ovary genetic background: C57BL/6 treatment: control
|
Treatment protocol |
Twenty-one C57BL/6 5 week old female mice were simultaneously exposed to cigarette smoke [twelve 3R4F reference cigarettes (University of Kentucky, USA) twice/day, 2.7 mg particulate matter, five times per week, for 12-18 weeks] using a custom-designed and purpose-built nose-only, directed flow inhalation and smoke-exposure system (CH Technologies, NJ, USA) housed in a fume and laminar flow hood. Each exposure lasted 60 minutes. Control mice received room air.
|
Growth protocol |
All experimental procedures involving the use of animals were performed with the approval of the University of Newcastle’s Animal Care and Ethics Committee (ACEC). Specific pathogen-free adult female (6-week-old) C57BL/6 mice were obtained from the animal services unit and maintained according to the recommendations prescribed by the ACEC. Mice were housed under a controlled lighting regime (16L:8D) at 21–22°C and supplied with food and water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA (approximately 3 µg) was isolated from adult ovaries using a QIAGEN RNeasy Mini Kit (Hilden, Germany) in accordance with the manufacturer’s recommendations.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
|
|
|
Submission date |
Dec 12, 2012 |
Last update date |
Dec 13, 2012 |
Contact name |
Alexander Peter Sobinoff |
E-mail(s) |
c3045119@uon.edu.au
|
Organization name |
University of Newcastle Australia
|
Department |
Science and IT
|
Lab |
Reproductive Science Group
|
Street address |
University Drive
|
City |
Newcastle |
State/province |
NSW |
ZIP/Postal code |
2308 |
Country |
Australia |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE42889 |
Expression data from adult mouse ovaries exposed to cigarette smoke in vivo |
|