|
| Status |
Public on Jul 28, 2006 |
| Title |
retroperitoneal adipose tissue, Day 2, Control Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse retroperitoneal fat pad
|
| Organism |
Mus musculus |
| Characteristics |
JMin_MG4302_051905_18_D2CTR
Genotype:C57BK/6J
Age: 9 wk old mice
|
| Treatment protocol |
Two retroperitoneal fat pads excised from each of 10 mice, pooled, frozen in liquid N2 and later extracted in Trizol solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 420 2.0 arrays using the Hybridization Oven 640, the hybridized array washed with the Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from retroperitoneal adipose tissue of C57BK/6J mice fed control diet.
|
| Data processing |
The data were analyzed with robust multi-array analysis RMA. The RMA signal values wre log2 transformed.
|
| |
|
| Submission date |
Apr 13, 2006 |
| Last update date |
Jul 28, 2006 |
| Contact name |
Chris LaRosa |
| E-mail(s) |
plarosa1@unl.edu
|
| Phone |
4024724198
|
| Fax |
402472-3139
|
| Organization name |
University of Nebraska, Lincoln
|
| Department |
Center of Biotechnology
|
| Street address |
19th & Vine
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588-0665 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4671 |
Microarray Analysis of the Delipidation of White Adipose Tissue of Mice Fed Conjugated Linoleic Acid |
|
