|
| Status |
Public on Jul 04, 2015 |
| Title |
E13.5 distal half of midgut-Id2 KO-1 |
| Sample type |
RNA |
| |
|
| Source name |
Embryo of littermate Id2 knock out mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: distal half of midgut
genotype/variation: Id2 KO
developmental stage: E13.5 embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Distal half of the midgut segments of Id2 WT and KO embryo were disected at E13.5. Total RNA of each samples were extarcted by RNeasyMicro Kit.
|
| Label |
Digoxigenin
|
| Label protocol |
One µg of total RNA was amplified with one round NanoAmpTM RT-IVT Labeling Kit (Applied Biosystems).
|
| |
|
| Hybridization protocol |
Digoxigenin-labeled cRNA samples were hybridized to Mouse Genome Survey Microarrays V2.0 (Applied Biosystems) at a temperature of 55℃. After several washing steps of increasing stringency, an anti-digoxigenin-antibody conjugated to alkaline phosphatase (Roche Diagnostics) was added.
|
| Scan protocol |
Chemiluminescence and fluorescence signals were detected on an AB1700 microarray reader. Reagents from the Chemiluminescence Detection Kit (Applied Biosystems) were used in this procedure.
|
| Data processing |
Detectable probes were determined by a signal to noise ratio (S/N) > 3 and quality flag < 5,000 determined by the AB1700 microarray software tool.
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
Jul 04, 2015 |
| Contact name |
Kentaro Mori |
| E-mail(s) |
kemori@u-fukui.ac.jp
|
| Phone |
+81-776-61-8313
|
| Fax |
+81-776-61-8164
|
| Organization name |
University of Fukui
|
| Street address |
23-3
|
| City |
Shimoaizuki, Matsuoka |
| State/province |
Fukui |
| ZIP/Postal code |
910-1193 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE43014 |
Gene expression profiles in the embryonic midgut of Id2 knockout and wild-type mice |
|
