|
| Status |
Public on May 01, 2013 |
| Title |
Ovcar_control_rep3_mouse |
| Sample type |
RNA |
| |
|
| Source name |
Control OVCAR3
|
| Organism |
Mus musculus |
| Characteristics |
strain: nude mice
cell type: Ovcar3
transfection: empty vector
|
| Growth protocol |
6-weeks-old male nude mice were challenged subcutaneously in the left flank with parental or RNASET2-silenced OVCAR3 cells. Animals were monitored twice a week for weight and tumor growth (measuring three perpendicular diameters), and killed at day 39. Separate experiments were performed with three different clones of both control (parental cells) and RNASET2-silenced cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were killed at day 39 and tumors were frozen for RNA extraction.Total RNA extracted using RNeasy Plus kit (Qiagen) according to the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
Scanned with the Agilent G2565BA Microarray Scanner (Agilent)
|
| Description |
Ovcar_control_rep3
Gene expression of OVCAR3 injected into mice
|
| Data processing |
The fluorescence intensities on scanned images were extracted and preprocessed by Agilent Feature Extraction Software (v10.5.1.1).
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
May 01, 2013 |
| Contact name |
Marco Fabbri |
| E-mail(s) |
fabbri.marco@gmail.com
|
| Organization name |
Università dell'Insubria
|
| Street address |
via dunant 5
|
| City |
Varese (VA) |
| ZIP/Postal code |
21100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE43029 |
RNASET2-silenced and control OVCAR3 cell clones injected into nude mice. |
|
