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Sample GSM1055350 Query DataSets for GSM1055350
Status Public on Sep 05, 2013
Title Hippocampus, Wild type, old (23 month)-5
Sample type RNA
 
Source name Hippocampus, Wild type, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: wild type
age: Old (23 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
 
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 9.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
 
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
E-mail(s) ryan.grant@pbrc.edu
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
ID_REF
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1753.144 0
ILMN_2721178 257.1011 0
ILMN_3033922 470.5638 0
ILMN_3092673 2985.47 0
ILMN_2816356 17.90543 0.03007519
ILMN_2808939 484.1177 0
ILMN_2634564 262.8036 0
ILMN_2737647 3.878451 0.2117794
ILMN_2734484 399.2458 0
ILMN_2952292 44.02085 0
ILMN_2699078 -2.012388 0.4862155
ILMN_1213681 201.506 0
ILMN_2735413 4.174776 0.2005012
ILMN_2735415 3.085239 0.2343358
ILMN_2891688 567.2529 0
ILMN_2637698 1203.071 0
ILMN_2674228 107.6168 0
ILMN_2601546 15.12659 0.05513784
ILMN_1230831 17.52679 0.03258145
ILMN_2848071 14.15449 0.05639098

Total number of rows: 25697

Table truncated, full table size 727 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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