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Sample GSM1055350 Query DataSets for GSM1055350
Status Public on Sep 05, 2013
Title Hippocampus, Wild type, old (23 month)-5
Sample type RNA
Source name Hippocampus, Wild type, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: wild type
age: Old (23 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 9.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1753.144 0
ILMN_2721178 257.1011 0
ILMN_3033922 470.5638 0
ILMN_3092673 2985.47 0
ILMN_2816356 17.90543 0.03007519
ILMN_2808939 484.1177 0
ILMN_2634564 262.8036 0
ILMN_2737647 3.878451 0.2117794
ILMN_2734484 399.2458 0
ILMN_2952292 44.02085 0
ILMN_2699078 -2.012388 0.4862155
ILMN_1213681 201.506 0
ILMN_2735413 4.174776 0.2005012
ILMN_2735415 3.085239 0.2343358
ILMN_2891688 567.2529 0
ILMN_2637698 1203.071 0
ILMN_2674228 107.6168 0
ILMN_2601546 15.12659 0.05513784
ILMN_1230831 17.52679 0.03258145
ILMN_2848071 14.15449 0.05639098

Total number of rows: 25697

Table truncated, full table size 727 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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