|
| Status |
Public on Sep 05, 2013 |
| Title |
Hippocampus, ASC -/-, old (23 month)-1 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus, ASC -/-, old (23 month)
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6J
genotype/variation: ASC -/-
age: Old (23 month)
tissue: Hippocampus
|
| Growth protocol |
Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
|
| |
|
| Hybridization protocol |
Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
15.AVG_Signal
|
| Data processing |
Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Ryan Grant |
| E-mail(s) |
ryan.grant@pbrc.edu
|
| Phone |
225-763-2763
|
| Fax |
225-763-0261
|
| Organization name |
Pennington Biomedical Research Center
|
| Lab |
Immunobiology
|
| Street address |
6400 Perkins Rd
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE43034 |
Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline |
|
