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Sample GSM1055357 Query DataSets for GSM1055357
Status Public on Sep 05, 2013
Title Hippocampus, ASC -/-, old (23 month)-3
Sample type RNA
Source name Hippocampus, ASC -/-, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: ASC -/-
age: Old (21 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 17.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1753.144 0
ILMN_2721178 276.0267 0
ILMN_3033922 444.3758 0
ILMN_3092673 2838.015 0
ILMN_2816356 17.10069 0.04636591
ILMN_2808939 508.801 0
ILMN_2634564 318.0438 0
ILMN_2737647 5.708592 0.1904762
ILMN_2734484 414.4482 0
ILMN_2952292 38.68709 0.002506266
ILMN_2699078 -3.295572 0.5739349
ILMN_1213681 195.8835 0
ILMN_2735413 -0.3795136 0.4072682
ILMN_2735415 7.077815 0.1654135
ILMN_2891688 520.5024 0
ILMN_2637698 1102.789 0
ILMN_2674228 97.9597 0
ILMN_2601546 3.970462 0.2343358
ILMN_1230831 7.91686 0.1491228
ILMN_2848071 22.15943 0.02631579

Total number of rows: 25697

Table truncated, full table size 734 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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