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Sample GSM1055360 Query DataSets for GSM1055360
Status Public on Sep 05, 2013
Title Hippocampus, ASC -/-, old (23 month)-6
Sample type RNA
 
Source name Hippocampus, ASC -/-, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: ASC -/-
age: Old (21 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
 
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 20.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
 
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
E-mail(s) ryan.grant@pbrc.edu
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
ID_REF
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1743.465 0
ILMN_2721178 307.9414 0
ILMN_3033922 473.5065 0
ILMN_3092673 2913.543 0
ILMN_2816356 19.42908 0.03007519
ILMN_2808939 464.1997 0
ILMN_2634564 312.252 0
ILMN_2737647 7.178276 0.1578947
ILMN_2734484 363.9154 0
ILMN_2952292 48.99096 0.001253133
ILMN_2699078 -0.5064205 0.4160401
ILMN_1213681 212.1748 0
ILMN_2735413 -5.129385 0.7142857
ILMN_2735415 7.579579 0.1478697
ILMN_2891688 454.1711 0
ILMN_2637698 1051.548 0
ILMN_2674228 114.8277 0
ILMN_2601546 10.04697 0.1115288
ILMN_1230831 3.485724 0.2431078
ILMN_2848071 24.64459 0.01879699

Total number of rows: 25697

Table truncated, full table size 734 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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