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Sample GSM1055364 Query DataSets for GSM1055364
Status Public on Sep 05, 2013
Title Hippocampus, CIAS -/-, old (23 month)-1
Sample type RNA
 
Source name Hippocampus, CIAS -/-, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: CIAS -/-
age: Old (23 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
 
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 25.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
 
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
E-mail(s) ryan.grant@pbrc.edu
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
ID_REF
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1765.728 0
ILMN_2721178 250.4637 0
ILMN_3033922 408.3958 0
ILMN_3092673 3105.168 0
ILMN_2816356 22.44586 0.02882206
ILMN_2808939 510.4499 0
ILMN_2634564 315.2753 0
ILMN_2737647 6.729486 0.179198
ILMN_2734484 429.777 0
ILMN_2952292 39.2571 0.002506266
ILMN_2699078 -5.556858 0.7431078
ILMN_1213681 222.2556 0
ILMN_2735413 -3.410699 0.5914787
ILMN_2735415 2.504014 0.3057644
ILMN_2891688 517.0956 0
ILMN_2637698 1168.389 0
ILMN_2674228 104.1362 0
ILMN_2601546 8.177092 0.1616541
ILMN_1230831 8.984327 0.141604
ILMN_2848071 16.29328 0.05513784

Total number of rows: 25697

Table truncated, full table size 732 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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