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Sample GSM1055367 Query DataSets for GSM1055367
Status Public on Sep 05, 2013
Title Hippocampus, CIAS -/-, old (23 month)-4
Sample type RNA
 
Source name Hippocampus, CIAS -/-, old (23 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: CIAS -/-
age: Old (23 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
 
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 28.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
 
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
E-mail(s) ryan.grant@pbrc.edu
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
ID_REF
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1774.171 0
ILMN_2721178 254.1137 0
ILMN_3033922 467.6903 0
ILMN_3092673 3381.42 0
ILMN_2816356 13.63834 0.07769424
ILMN_2808939 462.0335 0
ILMN_2634564 288.8511 0
ILMN_2737647 4.692828 0.2030075
ILMN_2734484 397.0688 0
ILMN_2952292 54.13268 0
ILMN_2699078 -2.575524 0.5388471
ILMN_1213681 159.5113 0
ILMN_2735413 -2.232454 0.5200501
ILMN_2735415 3.835861 0.2305764
ILMN_2891688 490.8163 0
ILMN_2637698 1051.102 0
ILMN_2674228 96.11588 0
ILMN_2601546 18.407 0.03508772
ILMN_1230831 7.719216 0.1466165
ILMN_2848071 17.46509 0.04010025

Total number of rows: 25697

Table truncated, full table size 731 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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