NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1055370 Query DataSets for GSM1055370
Status Public on Sep 05, 2013
Title Hippocampus, CASP 11-/-, young (1 month)-1
Sample type RNA
 
Source name Hippocampus, CASP 11-/-, young (1 month)
Organism Mus musculus
Characteristics background strain: C57BL/6J
genotype/variation: CASP11-/-
age: Young (1 month)
tissue: Hippocampus
Growth protocol Wild type and knockout mice were fed a chow diet after weaning for the entire duration of the experiment. Young mice were sacrificed at 1 month and old mice were sacrificed at 21-23 months.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, and then transferred to QIAGEN RNeasy columns and Dnase I treated according to the prescribed protocol provided with the kit. Quality control and RNA concentration were determined using Nanodrop and Agilent Bioanalyser.
Label biotin
Label protocol Epicentre TargetAmp Nano-g Biotin-aRNA Labeling Kit for Illumina (TAN07924)-Manufacturer's protocol 200ng input total RNA
 
Hybridization protocol Illumina Whole Genome Expression Direct Hybridization Assay Guide- 750 ng labeled aRNA hybridized to array
Scan protocol Standard Illumina scanning protocol
Description 31.AVG_Signal
Data processing Quantile normalization and background subtraction using Illumina GenomeStudio Gene Expression Module v1.9.0
 
Submission date Dec 19, 2012
Last update date Sep 05, 2013
Contact name Ryan Grant
E-mail(s) ryan.grant@pbrc.edu
Phone 225-763-2763
Fax 225-763-0261
Organization name Pennington Biomedical Research Center
Lab Immunobiology
Street address 6400 Perkins Rd
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platform ID GPL6885
Series (1)
GSE43034 Canonical Nlrp3 inflammasome activation links systemic age-related inflammation with functional decline

Data table header descriptions
ID_REF
VALUE Illumina GenomeStudio background subtracted and quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2399.226 0
ILMN_2721178 316.402 0
ILMN_3033922 472.713 0
ILMN_3092673 3093.958 0
ILMN_2816356 13.75383 0.06140351
ILMN_2808939 482.3899 0
ILMN_2634564 307.9414 0
ILMN_2737647 3.410242 0.2406015
ILMN_2734484 423.7094 0
ILMN_2952292 57.25419 0
ILMN_2699078 -2.170566 0.4987469
ILMN_1213681 194.6027 0
ILMN_2735413 0.4210695 0.3646617
ILMN_2735415 5.219483 0.2042606
ILMN_2891688 585.6912 0
ILMN_2637698 1151.551 0
ILMN_2674228 120.2418 0
ILMN_2601546 5.691792 0.1967418
ILMN_1230831 11.72267 0.0839599
ILMN_2848071 24.00304 0.02005013

Total number of rows: 25697

Table truncated, full table size 727 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap