|
| Status |
Public on Dec 30, 2013 |
| Title |
Chat_hb_ip_1 |
| Sample type |
RNA |
| |
|
| Source name |
Trap'd MHb cholinergic neuron RNA, biological replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Dissected habenula
age: adult (6-12 weeks)
strain: Chat DW167
sample type: TRAP RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRAP affinity purification of ribosomes from BAC transgenic mice(see PMID:19013282), followed by Trizol extraction, DNAse treatment, and RNeasy-Minelute Cleanup
|
| Label |
Biotin
|
| Label protocol |
Affymetrix two cycle amplification kit, starting with 20 ng of total RNA
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol for mouse 430 2.0 platform
|
| Scan protocol |
Standard Affymetrix protocol for mouse 430 2.0 platform
|
| Description |
Trap'd MHb cholinergic neuron RNA, biological replicate 1
|
| Data processing |
GCRMA normalization within biological replicate groups. Performed with R / Bioconductor. Entrez CDFs, version 14.
|
| |
|
| Submission date |
Dec 27, 2012 |
| Last update date |
Dec 30, 2013 |
| Contact name |
Joseph D Dougherty |
| Organization name |
Washington University
|
| Department |
Genetics and Psychiatry
|
| Street address |
4566 Scott Ave, box 8232
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL16225 |
| Series (1) |
| GSE43164 |
Relapse from nicotine abstinence increases the pacemaking frequency of cholinergic habenular neurons |
|
