|
| Status |
Public on Apr 20, 2006 |
| Title |
Fred Pep8 peritoneal mesothial vs. SO2274 (ExpID 2561) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Fred Pep8 peritoneal mesothial (Cy3)
|
| Organism |
Rattus norvegicus |
| Characteristics |
Fred Pep8 peritoneal mesothial
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
SO2274 (Cy5)
|
| Organism |
Rattus norvegicus |
| Characteristics |
SO2274
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
Following exposure to 2000 ppm of o-nitrotoluene (o-NT) for two years, total RNA from rat peritoneal mesotheliomas was compared to a non-transformed mesothelial cell line (Fred-PE). RNAs were isolated with the RNeasy kit according to the manufacturer's protocols.
|
| Data processing |
Five hundred ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol. For each two color comparison, 750 ng of each Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using defaults for all parameters.
|
| |
|
| Submission date |
Apr 18, 2006 |
| Last update date |
Jul 06, 2007 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL890 |
| Series (1) |
| GSE4682 |
Gene expression analysis of peritoneal mesotheliomas following chemical treatment in F344 rats |
|
