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Sample GSM105789 Query DataSets for GSM105789
Status Public on Apr 20, 2006
Title Fred Pep8 peritoneal mesothial vs. SO2122 (ExpID 2560)
Sample type RNA
 
Channel 1
Source name Fred Pep8 peritoneal mesothial (Cy3)
Organism Rattus norvegicus
Characteristics Fred Pep8 peritoneal mesothial
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name SO2122 (Cy5)
Organism Rattus norvegicus
Characteristics SO2122
Extracted molecule total RNA
Label Cy5
 
 
Description Following exposure to 2000 ppm of o-nitrotoluene (o-NT) for two years, total RNA from rat peritoneal mesotheliomas was compared to a non-transformed mesothelial cell line (Fred-PE). RNAs were isolated with the RNeasy kit according to the manufacturer's protocols.
Data processing Five hundred ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol. For each two color comparison, 750 ng of each Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using defaults for all parameters.
 
Submission date Apr 18, 2006
Last update date Jul 06, 2007
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL890
Series (1)
GSE4682 Gene expression analysis of peritoneal mesotheliomas following chemical treatment in F344 rats

Data table header descriptions
ID_REF
rProcessedSignal
gProcessSignal
VALUE Log10(Ratio of Treated:Control)

Data table
ID_REF rProcessedSignal gProcessSignal VALUE
1 1703.937 6086.324 -0.552901534443142
2 39.76568 20.32311 0.291518245553764
3 620.1479 648.9734 -0.0197316189928316
4 330.8446 382.6487 -0.0631761916653052
5 480.378 496.9315 -0.0147134174334858
6 696.4118 956.4508 -0.137796513559782
7 159.0682 5379.029 -1.52912051903533
8 655.151 700.3821 -0.0289936298320246
9 89.22584 50.92913 0.243524387853893
10 1580.677 1472.909 0.0306672181651222
11 118.8714 125.2667 -0.0227582589995638
12 360.5432 411.9123 -0.057847451504976
13 2131.952 2455.324 -0.0613313864271006
14 167.73 5581.665 -1.52215302049901
15 29856.12 38929.58 -0.115246350646151
16 598.4998 603.2289 -0.00341813029249521
17 706.6871 801.4305 -0.0546387026408653
18 97.37079 100.6129 -0.0142249734096955
19 1494.009 1449.723 0.0130681846156774
20 68.46566 97.60839 -0.154014350313673

Total number of rows: 21575

Table truncated, full table size 892 Kbytes.




Supplementary file Size Download File type/resource
GSM105789.tif.gz 26.1 Mb (ftp)(http) TIFF
GSM105789.txt.gz 5.0 Mb (ftp)(http) TXT

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