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Sample GSM1058035

Query DataSets for GSM1058035

Status

Public on Feb 15, 2013

Title

whole zebrafish 36hpf, glucose treated, biological replicate 2

Sample type

RNA

Source name

glucose treated

Organism

Danio rerio

Characteristics

treatment: 1% glucose

Treatment protocol

Embroys grown in 1% glucose in E3 media from 12-36hpf

Growth protocol

Incubated at 28C

Extracted molecule

total RNA

Extraction protocol

RNA was extracted with Trizol and submitted to the Microarray Core Facility at the Molecular Genetics Department at Children's Hospital Boston/ Harvard Medical School

Label

biotin

Label protocol

Affymetrix protocol

Hybridization protocol

Affymetrix protocol

Scan protocol

Affymetrix protocol

Data processing

GC-RMA was performed in R for normalization and background correction.

Submission date

Dec 28, 2012

Last update date

Feb 15, 2013

Contact name

Trista North

Organization name

BIDMC

Street address

3 Blackfan Circle

City

Boston

State/province

ZIP/Postal code

02115

Country

USA

Platform ID

GPL1319

Series (1)

GSE43186

Glucose Metabolism impacts the spatio-temporal onset and magnitude of HSC induction in vivo

Data table header descriptions
ID_REF
VALUE	Normalized gene level expression from GC-RMA

Data table
ID_REF	VALUE
Dr.25180.1.S1_at	7.398666
Dr.20067.1.S1_at	125.7807
Dr.21691.1.A1_at	195.1905
Dr.10914.1.A1_at	149.3337
Dr.12986.1.A1_a_at	395.4813
Dr.12986.1.A1_at	166.9389
Dr.7100.1.A1_at	253.5466
DrAffx.3.1.A1_at	1632.004
Dr.5752.1.S1_at	94.1172
Dr.8724.1.S1_at	446.2149
Dr.21859.1.A1_at	353.4875
Dr.18387.1.A1_x_at	113.6093
Dr.11557.1.A1_at	136.7513
Dr.17950.1.S1_at	1240.98
Dr.26409.1.A1_at	213.5603
Dr.20484.1.S1_at	1286.794
Dr.14514.1.S1_a_at	746.1304
Dr.7658.1.S1_at	741.7924
Dr.24487.1.A1_at	1326.683
Dr.17748.1.A1_at	285.5979

Total number of rows: 15617

Table truncated, full table size 391 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM1058035_1gluc1b.CEL.gz	1.9 Mb	(ftp)(http)	CEL
Processed data included within Sample table