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Status |
Public on Dec 29, 2012 |
Title |
Pancreatic islets_control_rep3 |
Sample type |
RNA |
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Source name |
Pancreatic islets
|
Organism |
Mus musculus |
Characteristics |
gender: Male strain: ICR cell type: Pancreatic islets disease status: control
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Treatment protocol |
Fathers type 2 diabetes vs control, mate with females in standard conditions, then the offspring's liver transcriptomics were analysed through microarray analysis.
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Growth protocol |
At 16 weeks of age, fathers of type 2 diabetes or control were mated with normal females in standard conditions, to generate the F1 offspring.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. (Invitrogen)
|
Label |
Cy3
|
Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
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Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
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Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
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Description |
This sample is the third of six control biological replicates used in this experiment, each from separate animals.
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
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Submission date |
Dec 28, 2012 |
Last update date |
Dec 29, 2012 |
Contact name |
yanchang wei |
E-mail(s) |
yanchangwei@yahoo.cn
|
Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
Street address |
#1 Beichen West Rd
|
City |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
|
|
Platform ID |
GPL10192 |
Series (2) |
GSE43187 |
Paternally induced transgenerational inheritance of susceptibility to diabetes in mammals (NimbleGen expression) |
GSE43239 |
Paternally induced transgenerational inheritance of susceptibility to diabetes in mammals |
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