|
| Status |
Public on Dec 29, 2012 |
| Title |
Pancreatic islets_t2d_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic islets
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: ICR
cell type: Pancreatic islets
disease status: t2d
|
| Treatment protocol |
Fathers type 2 diabetes vs control, mate with females in standard conditions, then the offspring's liver transcriptomics were analysed through microarray analysis.
|
| Growth protocol |
At 16 weeks of age, fathers of type 2 diabetes or control were mated with normal females in standard conditions, to generate the F1 offspring.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is the third of six experimental biological replicates used in this experiment, each from separate animals.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Dec 28, 2012 |
| Last update date |
Dec 29, 2012 |
| Contact name |
yanchang wei |
| E-mail(s) |
yanchangwei@yahoo.cn
|
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Street address |
#1 Beichen West Rd
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL10192 |
| Series (2) |
| GSE43187 |
Paternally induced transgenerational inheritance of susceptibility to diabetes in mammals (NimbleGen expression) |
| GSE43239 |
Paternally induced transgenerational inheritance of susceptibility to diabetes in mammals |
|
