|
| Status |
Public on Dec 31, 2012 |
| Title |
CCE_WT_Ctrl_2 |
| Sample type |
RNA |
| |
|
| Source name |
CCE_WT_Ctrl
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ESC
genotype/variation: CCE_WT
treatment: EtOH
time: 0hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent in accordance with the prescribed protocol. Quality control was performed with RNA 6000 NanoAssay and a Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
Total RNA from each sample was labeled using the Illumina Total Prep RNA Amplification kit (Ambion) and fragmented according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 2
replicate 2
|
| Data processing |
The data were normalized using quantile normalisation with GeneSpring 12.5 (Agilent)
|
| |
|
| Submission date |
Dec 31, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Kristian Bruun Laursen |
| E-mail(s) |
krl2004@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Pharmacology
|
| Lab |
Lorraine Gudas
|
| Street address |
1300 York Avenue
|
| City |
New York |
| ZIP/Postal code |
NY 10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE43221 |
Transcript levels in CCE WT and RARgamma knockout murine embryonic stem cells treated with either all-trans retinoic acid (8 and 24 hr) or with vehicle control |
|
