|
Status |
Public on Dec 31, 2012 |
Title |
CCE_WT_RA_8hr_1 |
Sample type |
RNA |
|
|
Source name |
CCE_WT_RA_8hr
|
Organism |
Mus musculus |
Characteristics |
cell type: ESC genotype/variation: CCE_WT treatment: RA time: 8hr
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent in accordance with the prescribed protocol. Quality control was performed with RNA 6000 NanoAssay and a Bioanalyzer 2100 (Agilent).
|
Label |
biotin
|
Label protocol |
Total RNA from each sample was labeled using the Illumina Total Prep RNA Amplification kit (Ambion) and fragmented according to the manufacturer’s instructions.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
SAMPLE 7 replicate 1
|
Data processing |
The data were normalized using quantile normalisation with GeneSpring 12.5 (Agilent)
|
|
|
Submission date |
Dec 31, 2012 |
Last update date |
Dec 31, 2012 |
Contact name |
Kristian Bruun Laursen |
E-mail(s) |
krl2004@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Pharmacology
|
Lab |
Lorraine Gudas
|
Street address |
1300 York Avenue
|
City |
New York |
ZIP/Postal code |
NY 10065 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE43221 |
Transcript levels in CCE WT and RARgamma knockout murine embryonic stem cells treated with either all-trans retinoic acid (8 and 24 hr) or with vehicle control |
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