|
Status |
Public on Apr 23, 2013 |
Title |
Sox17(+) 1 |
Sample type |
RNA |
|
|
Source name |
ES cells
|
Organism |
Mus musculus |
Characteristics |
treatment: Sox17-V5 expression cell line: KH2
|
Treatment protocol |
Doxycycline
|
Growth protocol |
mouse ES cells culture condtion
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Replicate 1
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Jan 02, 2013 |
Last update date |
Apr 23, 2013 |
Contact name |
Irene Aksoy |
E-mail(s) |
irene.aksoy@inserm.fr
|
Organization name |
Stem Cell and Brain Research Institute
|
Street address |
18 avenue du Doyen Lepine
|
City |
Lyon |
ZIP/Postal code |
69500 |
Country |
France |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE43233 |
Gene expression analysis of V5 tagged Sox17 expressing ES cells |
GSE43234 |
Sox7 and Sox17 |
|