|
| Status |
Public on Apr 23, 2013 |
| Title |
Sox17(+) 5 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Sox17-V5 expression
cell line: KH2
|
| Treatment protocol |
Doxycycline
|
| Growth protocol |
mouse ES cells culture condtion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jan 02, 2013 |
| Last update date |
Apr 23, 2013 |
| Contact name |
Irene Aksoy |
| E-mail(s) |
irene.aksoy@inserm.fr
|
| Organization name |
Stem Cell and Brain Research Institute
|
| Street address |
18 avenue du Doyen Lepine
|
| City |
Lyon |
| ZIP/Postal code |
69500 |
| Country |
France |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE43233 |
Gene expression analysis of V5 tagged Sox17 expressing ES cells |
| GSE43234 |
Sox7 and Sox17 |
|
