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Sample GSM1060251 Query DataSets for GSM1060251
Status Public on Jan 05, 2013
Title C1
Sample type RNA
 
Source name RAW264.7 cells, vehicle
Organism Mus musculus
Characteristics cell line: RAW264.7
cell type: macrophages
treatment: vehicle
treatment duration: overnight
Treatment protocol RAW264.7 cells were treated with vehicle, or LPS (100ng/ml) in the presence or absence of 1,25(OH)2D3 (20 nM) overnight.
Extracted molecule total RNA
Extraction protocol Total cellular RNAs were extracted from the cell lysates using TRIzol reagents (Invitrogen, Carlsbad, CA).
Label Hy3
Label protocol Using the miRCURY™ LNA Array Power labeling kit according to the manufacturer's protocol.
 
Hybridization protocol Using Tecan hybridization stations according to the manufacturer's protocol.
Scan protocol The arrays were scanned with a GenePix 4000B scanner using the manufacturer's recommended settings.
Description 14125704
Array covering Sanger miRBase 12.0 and Exiqon miRPlus mature miRNAs.
Data processing The raw data was extracted using GenePix Pro and imported into GeneSpring GX10 for analyses. Raw data were normalized using VSN normalization. The normalized data are log2 transformed. The Sample data table includes data for only mouse miRNA probes.
 
Submission date Jan 04, 2013
Last update date Jan 05, 2013
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL7723
Series (1)
GSE43300 1,25-Dihydroxyvitamin D Promotes Negative Feedback Regulation of Toll-Like Receptor Signaling via Targeting MicroRNA-155-SOCS1 in Macrophages

Data table header descriptions
ID_REF
VALUE Log2 normalized signal intensity

Data table
ID_REF VALUE
4040 7.417177226
4390 7.158352656
4610 6.801492982
4700 7.723837354
5730 6.742397215
5740 11.32990095
6880 7.171410845
10138 6.576732441
10306 9.165794045
10916 6.971585055
10919 8.306068898
10925 7.057973123
10928 8.981712743
10936 8.519590812
10937 6.589787861
10942 7.323870108
10943 7.035937196
10944 6.383699265
10946 6.890901997
10947 11.58028505

Total number of rows: 578

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM1060251_14125704.gpr.gz 399.3 Kb (ftp)(http) GPR
Processed data included within Sample table

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