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Sample GSM1060253 Query DataSets for GSM1060253
Status Public on Jan 05, 2013
Title LPS+VD3-1
Sample type RNA
 
Source name RAW264.7 cells, LPS+VD3
Organism Mus musculus
Characteristics cell line: RAW264.7
cell type: macrophages
treatment: lipopolysaccharide (LPS) + 1,25-dihydroxyvitamin D (VD3)
treatment duration: overnight
Treatment protocol RAW264.7 cells were treated with vehicle, or LPS (100ng/ml) in the presence or absence of 1,25(OH)2D3 (20 nM) overnight.
Extracted molecule total RNA
Extraction protocol Total cellular RNAs were extracted from the cell lysates using TRIzol reagents (Invitrogen, Carlsbad, CA).
Label Hy3
Label protocol Using the miRCURY™ LNA Array Power labeling kit according to the manufacturer's protocol.
 
Hybridization protocol Using Tecan hybridization stations according to the manufacturer's protocol.
Scan protocol The arrays were scanned with a GenePix 4000B scanner using the manufacturer's recommended settings.
Description 14125706
Array covering Sanger miRBase 12.0 and Exiqon miRPlus mature miRNAs.
Data processing The raw data was extracted using GenePix Pro and imported into GeneSpring GX10 for analyses. Raw data were normalized using VSN normalization. The normalized data are log2 transformed. The Sample data table includes data for only mouse miRNA probes.
 
Submission date Jan 04, 2013
Last update date Jan 05, 2013
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL7723
Series (1)
GSE43300 1,25-Dihydroxyvitamin D Promotes Negative Feedback Regulation of Toll-Like Receptor Signaling via Targeting MicroRNA-155-SOCS1 in Macrophages

Data table header descriptions
ID_REF
VALUE Log2 normalized signal intensity

Data table
ID_REF VALUE
4040 7.198505087
4390 7.083075016
4610 6.519241068
4700 7.562490746
5730 6.979902965
5740 12.54775813
6880 7.083996271
10138 6.630144798
10306 10.77145457
10916 6.773358898
10919 8.078560699
10925 7.019679641
10928 9.46035698
10936 8.176711143
10937 6.610444799
10942 7.273062132
10943 6.95247065
10944 6.524719532
10946 6.898187154
10947 12.04672331

Total number of rows: 578

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM1060253_14125706.gpr.gz 395.1 Kb (ftp)(http) GPR
Processed data included within Sample table

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