|
| Status |
Public on Jan 05, 2013 |
| Title |
LPS+VD3-2 |
| Sample type |
RNA |
| |
|
| Source name |
RAW264.7 cells, LPS+VD3
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW264.7
cell type: macrophages
treatment: lipopolysaccharide (LPS) + 1,25-dihydroxyvitamin D (VD3)
treatment duration: overnight
|
| Treatment protocol |
RAW264.7 cells were treated with vehicle, or LPS (100ng/ml) in the presence or absence of 1,25(OH)2D3 (20 nM) overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNAs were extracted from the cell lysates using TRIzol reagents (Invitrogen, Carlsbad, CA).
|
| Label |
Hy3
|
| Label protocol |
Using the miRCURY™ LNA Array Power labeling kit according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Using Tecan hybridization stations according to the manufacturer's protocol.
|
| Scan protocol |
The arrays were scanned with a GenePix 4000B scanner using the manufacturer's recommended settings.
|
| Description |
14125718
Array covering Sanger miRBase 12.0 and Exiqon miRPlus mature miRNAs.
|
| Data processing |
The raw data was extracted using GenePix Pro and imported into GeneSpring GX10 for analyses. Raw data were normalized using VSN normalization. The normalized data are log2 transformed. The Sample data table includes data for only mouse miRNA probes.
|
| |
|
| Submission date |
Jan 04, 2013 |
| Last update date |
Jan 05, 2013 |
| Contact name |
Yong Huang |
| E-mail(s) |
yh9fj@virginia.edu
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE43300 |
1,25-Dihydroxyvitamin D Promotes Negative Feedback Regulation of Toll-Like Receptor Signaling via Targeting MicroRNA-155-SOCS1 in Macrophages |
|
