|
| Status |
Public on Jan 08, 2013 |
| Title |
Spleen_B10_TBI_300cGy_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
total body irradiation dose: 300 cGy
strain: B10.BR
|
| Treatment protocol |
Mice were treated with total body irradiation using an XRAD320 Biological Irradiator (Precision X-Ray, North Branford, CT).
|
| Growth protocol |
Total RNA was extracted from the spleens immediately after harvesting from mice 24 hours after total body irradiation, or from resting mice for un-irradiated controls.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole splenocytes homogenized with a rotor-stator device in TRIzol (Invitrogen) followed by RNeasy purification. Samples quality was checked using an Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Agilent One-Color Quick Amp Labeling Protocol
|
| |
|
| Hybridization protocol |
Agilent One-color Heterologous GE Hybridization and Wash Protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 5 um resolution.
|
| Description |
TBI - 300 cGy
|
| Data processing |
Array images were processed with Agilent Feature Extraction software version 10.5.1.1. Array data files were filtered to remove positive and negative control features and any features flagged as non-uniform outliers or population outliers, as recommended by Agilent. Log-transformed signal intensity values were then globally normalized using the percentile shift algorithm, shifting to the 75th percentile of each sample, followed by baseline shifting to the median of all samples.
|
| |
|
| Submission date |
Jan 07, 2013 |
| Last update date |
Jan 08, 2013 |
| Contact name |
Thai Cao |
| E-mail(s) |
thai.cao@hsc.utah.edu
|
| Organization name |
University of Utah
|
| Street address |
30 North 1900 East, SOM5C402
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84108 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE43310 |
Gene expression profile in spleens of mice 24 hours after total body irradiation. |
|
