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Sample GSM1060735 Query DataSets for GSM1060735
Status Public on Jun 12, 2013
Title Skin
Sample type RNA
 
Source name Normal skin
Organism Homo sapiens
Characteristics tissue: Normal skin
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Label biotin
Label protocol In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
 
Hybridization protocol 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Scan protocol Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Description Normal skin
Data processing Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm with target intensity=100.
 
Submission date Jan 08, 2013
Last update date Jun 12, 2013
Contact name Atsushi Kaneda
E-mail(s) kaneda@genome.rcast.u-tokyo.ac.jp
Organization name The University of Tokyo
Department RCAST
Lab Genome Science Division
Street address 4-6-1 Komaba
City Meguro-ku
State/province Tokyo
ZIP/Postal code 153-8904
Country Japan
 
Platform ID GPL570
Series (2)
GSE43346 Gene repression with H3K27me3 modification in human small cell lung cancer
GSE99316 Gene repression and ChIP-seq in Human Small Cell Lung Cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity with target intensity=100

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.9
AFFX-BioB-M_at 1.1
AFFX-BioB-3_at 1.8
AFFX-BioC-5_at 752.9
AFFX-BioC-3_at 746
AFFX-BioDn-5_at 1328.8
AFFX-BioDn-3_at 3396.2
AFFX-CreX-5_at 10182.3
AFFX-CreX-3_at 10734.5
AFFX-DapX-5_at 3.9
AFFX-DapX-M_at 16.8
AFFX-DapX-3_at 2.7
AFFX-LysX-5_at 2
AFFX-LysX-M_at 2
AFFX-LysX-3_at 1.9
AFFX-PheX-5_at 9.5
AFFX-PheX-M_at 2.3
AFFX-PheX-3_at 9.4
AFFX-ThrX-5_at 6.1
AFFX-ThrX-M_at 3

Total number of rows: 54675

Table truncated, full table size 836 Kbytes.




Supplementary file Size Download File type/resource
GSM1060735_Skin_U133p2.CEL.gz 7.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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