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Sample GSM106142 Query DataSets for GSM106142
Status Public on Apr 30, 2006
Title myoblast undifferentiated C2C12, biological rep1
Sample type RNA
Source name C2C12 cells in 20% serum
Organism Mus musculus
Characteristics Adult muscle myoblast
Treatment protocol cells were risned once with PBS and then directly lysed in TriZol (Invitrogen).
Growth protocol 20% serum in MEM
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from undifferentiated C2C12 cells.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Apr 20, 2006
Last update date Apr 20, 2006
Contact name Ihsiung Brandon Chen
Organization name The Scripps Research Institute
Department Cell Biology
Lab Larry Gerace
Street address 10550 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platform ID GPL1261
Series (1)
GSE4694 Expression data from myogenesis

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 389.7 P 0.00039
AFFX-BioB-M_at 706.6 P 0.000044
AFFX-BioB-3_at 389.2 P 0.00034
AFFX-BioC-5_at 1149.9 P 0.00007
AFFX-BioC-3_at 932.7 P 0.000044
AFFX-BioDn-5_at 1404.2 P 0.000044
AFFX-BioDn-3_at 6296.1 P 0.000044
AFFX-CreX-5_at 12382.5 P 0.000044
AFFX-CreX-3_at 18704.2 P 0.000044
AFFX-DapX-5_at 19.8 A 0.659339
AFFX-DapX-M_at 14.3 A 0.737173
AFFX-DapX-3_at 6.6 A 0.852061
AFFX-LysX-5_at 87 P 0.039661
AFFX-LysX-M_at 4.4 A 0.945787
AFFX-LysX-3_at 39.3 A 0.262827
AFFX-PheX-5_at 4.1 A 0.969035
AFFX-PheX-M_at 3.6 A 0.973889
AFFX-PheX-3_at 16.2 A 0.672921
AFFX-ThrX-5_at 5.9 A 0.90441
AFFX-ThrX-M_at 3.1 A 0.984817

Total number of rows: 45101

Table truncated, full table size 1203 Kbytes.

Supplementary data files not provided

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