|
| Status |
Public on Apr 30, 2006 |
| Title |
myoblast undifferentiated C2C12, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cells in 20% serum
|
| Organism |
Mus musculus |
| Characteristics |
Adult muscle myoblast
Undifferentiated
|
| Treatment protocol |
cells were risned once with PBS and then directly lysed in TriZol (Invitrogen).
|
| Growth protocol |
20% serum in MEM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from undifferentiated C2C12 cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 20, 2006 |
| Last update date |
Apr 20, 2006 |
| Contact name |
Ihsiung Brandon Chen |
| E-mail(s) |
IBCHEN@YAHOO.COM
|
| Organization name |
The Scripps Research Institute
|
| Department |
Cell Biology
|
| Lab |
Larry Gerace
|
| Street address |
10550 N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4694 |
Expression data from myogenesis |
|
