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Sample GSM106145 Query DataSets for GSM106145
Status Public on Apr 30, 2006
Title myotubes, differentiated C2C12, biological rep1
Sample type RNA
 
Source name C2C12 cells in 2% horse serum
Organism Mus musculus
Characteristics Adult muscle myotubes
Differentiated
Treatment protocol cells were risned once with PBS and then directly lysed in TriZol (Invitrogen).
Growth protocol 2% horse serum in MEM
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from differentiated C2C12 cells on Day 6.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Apr 20, 2006
Last update date Apr 20, 2006
Contact name Ihsiung Brandon Chen
E-mail(s) IBCHEN@YAHOO.COM
Organization name The Scripps Research Institute
Department Cell Biology
Lab Larry Gerace
Street address 10550 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL1261
Series (1)
GSE4694 Expression data from myogenesis

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 474.1 P 0.000662
AFFX-BioB-M_at 871.5 P 0.00007
AFFX-BioB-3_at 312.3 P 0.000095
AFFX-BioC-5_at 1202.6 P 0.000052
AFFX-BioC-3_at 1284.6 P 0.000052
AFFX-BioDn-5_at 1574 P 0.000044
AFFX-BioDn-3_at 6871.3 P 0.000044
AFFX-CreX-5_at 15167.1 P 0.000044
AFFX-CreX-3_at 21093.5 P 0.000044
AFFX-DapX-5_at 29 A 0.205732
AFFX-DapX-M_at 47.2 A 0.659352
AFFX-DapX-3_at 6.6 A 0.699409
AFFX-LysX-5_at 26.1 A 0.123572
AFFX-LysX-M_at 9.2 A 0.712257
AFFX-LysX-3_at 5 A 0.659339
AFFX-PheX-5_at 11.9 A 0.686277
AFFX-PheX-M_at 2.9 A 0.957052
AFFX-PheX-3_at 18.2 A 0.48511
AFFX-ThrX-5_at 23.5 A 0.300606
AFFX-ThrX-M_at 37.3 A 0.216524

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.




Supplementary data files not provided

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