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Sample GSM106146 Query DataSets for GSM106146
Status Public on Apr 30, 2006
Title myotubes, differentiated C2C12, biological rep2
Sample type RNA
Source name C2C12 cells in 2% horse serum
Organism Mus musculus
Characteristics Adult muscle myotubes
Treatment protocol cells were risned once with PBS and then directly lysed in TriZol (Invitrogen).
Growth protocol 2% horse serum in MEM
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from differentiated C2C12 cells on Day 6.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Apr 20, 2006
Last update date Apr 20, 2006
Contact name Ihsiung Brandon Chen
Organization name The Scripps Research Institute
Department Cell Biology
Lab Larry Gerace
Street address 10550 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platform ID GPL1261
Series (1)
GSE4694 Expression data from myogenesis

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 572.4 P 0.000972
AFFX-BioB-M_at 1018 P 0.000081
AFFX-BioB-3_at 534.1 P 0.000297
AFFX-BioC-5_at 1399.7 P 0.00006
AFFX-BioC-3_at 1532.7 P 0.000044
AFFX-BioDn-5_at 1795.9 P 0.000052
AFFX-BioDn-3_at 8575 P 0.000044
AFFX-CreX-5_at 17615.5 P 0.000044
AFFX-CreX-3_at 24231.4 P 0.000044
AFFX-DapX-5_at 30.3 A 0.5
AFFX-DapX-M_at 11.8 A 0.672921
AFFX-DapX-3_at 47 A 0.205732
AFFX-LysX-5_at 6.5 A 0.544587
AFFX-LysX-M_at 6.5 A 0.921998
AFFX-LysX-3_at 43.6 P 0.026111
AFFX-PheX-5_at 6.5 A 0.834139
AFFX-PheX-M_at 3.6 A 0.978098
AFFX-PheX-3_at 13.1 A 0.686277
AFFX-ThrX-5_at 7 A 0.916408
AFFX-ThrX-M_at 8.5 A 0.686277

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided

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