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Sample GSM106147 Query DataSets for GSM106147
Status Public on Apr 30, 2006
Title myotubes, differentiated C2C12, biological rep3
Sample type RNA
Source name C2C12 cells in 2% horse serum
Organism Mus musculus
Characteristics Adult muscle myotubes
Treatment protocol cells were risned once with PBS and then directly lysed in TriZol (Invitrogen).
Growth protocol 2% horse serum in MEM
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA.
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from differentiated C2C12 cells on Day 6.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Apr 20, 2006
Last update date Apr 20, 2006
Contact name Ihsiung Brandon Chen
Organization name The Scripps Research Institute
Department Cell Biology
Lab Larry Gerace
Street address 10550 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platform ID GPL1261
Series (1)
GSE4694 Expression data from myogenesis

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 563.3 P 0.000857
AFFX-BioB-M_at 1292.8 P 0.000052
AFFX-BioB-3_at 613.7 P 0.000225
AFFX-BioC-5_at 1418 P 0.000044
AFFX-BioC-3_at 1684.4 P 0.000052
AFFX-BioDn-5_at 1803.3 P 0.000044
AFFX-BioDn-3_at 7734.3 P 0.000044
AFFX-CreX-5_at 17372.7 P 0.000044
AFFX-CreX-3_at 23104.9 P 0.000044
AFFX-DapX-5_at 19.1 A 0.51489
AFFX-DapX-M_at 54.6 A 0.52976
AFFX-DapX-3_at 7.6 A 0.760937
AFFX-LysX-5_at 8.2 A 0.327079
AFFX-LysX-M_at 9.6 A 0.772364
AFFX-LysX-3_at 6.6 A 0.617401
AFFX-PheX-5_at 5.1 A 0.984817
AFFX-PheX-M_at 6 A 0.783476
AFFX-PheX-3_at 29.2 A 0.617401
AFFX-ThrX-5_at 45 P 0.036557
AFFX-ThrX-M_at 5.4 A 0.772364

Total number of rows: 45101

Table truncated, full table size 1206 Kbytes.

Supplementary data files not provided

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