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Sample GSM1063688 Query DataSets for GSM1063688
Status Public on Jan 15, 2013
Title mammary tumor after orthotopic injection of 6dt1 cells expressing negative control rep2
Sample type RNA
 
Source name mammary tumor
Organism Mus musculus
Characteristics strain: FVB/N
tissue: mammary tumor
treatment: orthotopic injection of 6dt1 cells expressing negative control
Treatment protocol not applicable
Growth protocol Mammary tumors were collected 30 days after orthotopic injection of 6dt1 cells.
Extracted molecule total RNA
Extraction protocol Samples were snap frozen in liquid N2. Total RNA was isolated using RNAeasy Kit (Qiagen). RNA (100 ng) was reverse transcribed and amplified using WT Expression Kit (Ambion).
Label Affymetrix proprietary DNA Labeling Reagent that is covalently linked to biotin.
Label protocol Samples were enzymatically fragmented and biotinylated using the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized in the GeneChip Hybridization Oven 645 while shaking at 60 rpm at 45C for 16 hrs.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description Mammary tumor RNA collected 30 days after orthotopic injection of 6dt1 cells in female FVB/N mice.
Data processing Data were collected using the GeneChip Command Console Software (AGCC).
MoEx-1_0-st-v1.r2.pgf
MoGene-1_0-st-v1.na32.mm9.transcript
 
Submission date Jan 14, 2013
Last update date Jan 15, 2013
Contact name Natalie Goldberger
Organization name NCI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10740
Series (2)
GSE43485 Expression profiling in miR-290 6dt1 mammary tumors
GSE43487 Mammary tumors

Data table header descriptions
ID_REF
VALUE log2 RMA normalized gene level expression values from AGCC

Data table
ID_REF VALUE
10338001 10.66
10338002 6.55
10338003 9.14
10338004 7.73
10338005 3.28
10338006 3.53
10338007 3.82
10338008 4.4
10338009 8.55
10338010 3.31
10338011 6
10338012 3.45
10338013 3.14
10338014 3.17
10338015 3.15
10338016 7.87
10338017 11.5
10338018 6.98
10338019 5.53
10338020 8.46

Total number of rows: 241576

Table truncated, full table size 3313 Kbytes.




Supplementary file Size Download File type/resource
GSM1063688_Negative_B_6dt1_Tumor.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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