|
Status |
Public on Apr 27, 2006 |
Title |
fibroblast_unsorted_control_rep1 |
Sample type |
RNA |
|
|
Source name |
primary dermal fibroblasts untreated unsorted
|
Organism |
Mus musculus |
Characteristics |
strain: B6; gender: female; age: 0-2d; tissue: dermis; passage #: 4; treatment: untreated; biological replicate: 1; sort: unsorted
|
Treatment protocol |
untreated
|
Growth protocol |
Cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM;Invitrogen; Carlsbad, CA, USA) with L-glutamine and high glucose plus 10% FBS (Invitrogen) and 100U/mL penicillin streptomycin (Invitrogen) at 37oC and 5% CO 2. Cells were passaged at confluence by 0.25% trypsin EDTA (Invitrogen).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy total RNA extraction protocol (Qiagen)
|
Label |
biotin labeled with standard Affymetrix fluorescent stain cocktail
|
Label protocol |
standard Affymetrix IVT labeling and staining protocols
|
|
|
Hybridization protocol |
standard Affymetrix hybridization protocol
|
Scan protocol |
standard Affymetrix scan protocol
|
Description |
X chromosome-linked GFP heterozygous female mice were used to derive dermal fibroblasts. Fibroblasts were sorted through FACS for GFP-nonexpressing cells. Cells were then untreated as a control for 48 hours followed by 24 hour recovery in normal medium.; biological replicate 1
|
Data processing |
MAS 5.0 data processing and normalization
|
|
|
Submission date |
Apr 24, 2006 |
Last update date |
Apr 26, 2006 |
Contact name |
Tammy Vallender |
E-mail(s) |
tfw@uchicago.edu
|
Phone |
7738344065
|
Organization name |
University of Chicago
|
Department |
Human Genetics
|
Lab |
Dr. Bruce Lahn
|
Street address |
929 E 57th St W503V
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE4718 |
Improved DNA methylation analysis via enrichment of demethylated cells expressing an X-inactivated GFP transgene |
|