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Sample GSM1067687 Query DataSets for GSM1067687
Status Public on Apr 01, 2013
Title Negative control shRNA transduced cells, treated with 10µM isoproterenol for 3 hrs, replicate 1
Sample type RNA
Source name Negative control shRNA transduced cells, treated with 10µM isoproterenol for 3 hrs
Organism Mus musculus
Characteristics cell type: 3T3-L1 adipocytes
vector: Control shRNA
treatment: 10µM isoproterenol, 3 hrs
Treatment protocol Mature adipocytes 9 days after induction of differentiation were transduced with adenoviruses carrying shRNA against TBLR1 or a control sequence at a multiplicity of infection (MOI) of 500. After 3 days, cells were preincubated in Krebs-Ringer buffer for 2 hrs, then cells were stimulated with 10 µM isoproterenol for 3 hrs.
Growth protocol 3T3-L1 preadipocytes were cultured in low (1g/l) glucose DMEM medium containing 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S) and differentiated into mature white adipocytes two days post confluency by the addition of 1 µg/ml insulin, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 0.25 µM dexamethasone and 1/1000 volume ABP (50 mg/ml L-ascorbate, 1 mM biotin, 17 mM pantothenate) in high (4.5 g/l) glucose DMEM containing 10% FCS and 1% P/S
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Quiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from 3T3-L1 adipocytes treated with negative control shRNA and 10µM isoproterenol for 3 hrs
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
Submission date Jan 22, 2013
Last update date Apr 01, 2013
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL14661
Series (1)
GSE43658 Transcriptional co-factor TBLR1 controls lipid mobilization in white adipose tissue

Data table header descriptions
VALUE quantile normalized signal

Data table
AFFX-BioB-5_at 8.972656
AFFX-BioB-M_at 9.548828
AFFX-BioB-3_at 9.287109
AFFX-BioC-5_at 10.1582
AFFX-BioC-3_at 10.5293
AFFX-BioDn-5_at 11.25781
AFFX-BioDn-3_at 12.5332
AFFX-CreX-5_at 13.71094
AFFX-CreX-3_at 13.9668
AFFX-DapX-5_at 8.251953
AFFX-DapX-M_at 9.519531
AFFX-DapX-3_at 10.24609
AFFX-LysX-5_at 6.5625
AFFX-LysX-M_at 6.935547
AFFX-LysX-3_at 7.608398
AFFX-PheX-5_at 7.166016
AFFX-PheX-M_at 7.166016
AFFX-PheX-3_at 7.834961
AFFX-ThrX-5_at 6.825195
AFFX-ThrX-M_at 7.21582

Total number of rows: 16553

Table truncated, full table size 294 Kbytes.

Supplementary file Size Download File type/resource
GSM1067687_Liebert_290910_7-b-3T3L1-shNC+10uM-Iso_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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