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Sample GSM1067692 Query DataSets for GSM1067692
Status Public on Apr 01, 2013
Title TBLR1 specific shRNA transduced cells, treated with 10µM isoproterenol for 3 hrs, replicate 3
Sample type RNA
 
Source name TBLR1 specific shRNA transduced cells, treated with 10µM isoproterenol for 3 hrs
Organism Mus musculus
Characteristics cell type: 3T3-L1 adipocytes
vector: TBLR1 shRNA
treatment: 10µM isoproterenol, 3 hrs
Treatment protocol Mature adipocytes 9 days after induction of differentiation were transduced with adenoviruses carrying shRNA against TBLR1 or a control sequence at a multiplicity of infection (MOI) of 500. After 3 days, cells were preincubated in Krebs-Ringer buffer for 2 hrs, then cells were stimulated with 10 µM isoproterenol for 3 hrs.
Growth protocol 3T3-L1 preadipocytes were cultured in low (1g/l) glucose DMEM medium containing 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S) and differentiated into mature white adipocytes two days post confluency by the addition of 1 µg/ml insulin, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 0.25 µM dexamethasone and 1/1000 volume ABP (50 mg/ml L-ascorbate, 1 mM biotin, 17 mM pantothenate) in high (4.5 g/l) glucose DMEM containing 10% FCS and 1% P/S
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Quiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from 3T3-L1 adipocytes treated with TBLR1 specific shRNA and 10µM isoproterenol for 3 hrs
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Jan 22, 2013
Last update date Apr 01, 2013
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE43658 Transcriptional co-factor TBLR1 controls lipid mobilization in white adipose tissue

Data table header descriptions
ID_REF
VALUE quantile normalized signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.751953
AFFX-BioB-M_at 9.300781
AFFX-BioB-3_at 9.013672
AFFX-BioC-5_at 9.960938
AFFX-BioC-3_at 10.3457
AFFX-BioDn-5_at 11.06055
AFFX-BioDn-3_at 12.36914
AFFX-CreX-5_at 13.72266
AFFX-CreX-3_at 13.92383
AFFX-DapX-5_at 8.113281
AFFX-DapX-M_at 9.378906
AFFX-DapX-3_at 10.16016
AFFX-LysX-5_at 6.75
AFFX-LysX-M_at 6.946289
AFFX-LysX-3_at 7.428711
AFFX-PheX-5_at 7.130859
AFFX-PheX-M_at 7.189453
AFFX-PheX-3_at 7.698242
AFFX-ThrX-5_at 6.783203
AFFX-ThrX-M_at 7.097656

Total number of rows: 16553

Table truncated, full table size 294 Kbytes.




Supplementary file Size Download File type/resource
GSM1067692_Liebert_290910_12-d-3T3L1-shTBLR+10uM-Iso_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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