|
| Status |
Public on May 01, 2013 |
| Title |
ob_16 weeks_fasted_rep_3 [muscle] |
| Sample type |
RNA |
| |
|
| Source name |
muscle
|
| Organism |
Mus musculus |
| Characteristics |
age: 16 weeks
fasting status: fasted
strain: B6.V-Lep ob/J
diet: D12450B
tissue: muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: 06M
|
| Data processing |
The data were quantile normalised using lumi library in R
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Krzysztof Goryca |
| Organization name |
Uniwersytet Warszawski
|
| Department |
CeNT
|
| Street address |
Banacha 2c
|
| City |
Warszawa |
| ZIP/Postal code |
02-097 |
| Country |
Poland |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE43691 |
Hepatic molecular alterations more than muscle's differentiate obese hyperphagic mice from those prolonged fed with the high-fat diet |
|
