periaqueductal gray regions were dissected from two A/J mice under a dissection microscope. The samples were snap frozen and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
Label
biotin
Label protocol
10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type 8 wk old male A/J mouse periaqueductal gray
