|
| Status |
Public on Dec 09, 2013 |
| Title |
Untreated 2hpf embryo RNA expression |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
pooled whole zebrafish embryo 2hpf_untreated
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole embryo
developmental stage: 2 hr post fertilization
treated with: none (untreated)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol following manufactors specifications
|
| Label |
Cy3
|
| Label protocol |
Followed Agilent protocol substituting COT DNA with salmon sperm DNA at 10ug/ul
|
| |
|
| Channel 2 |
| Source name |
pooled whole zebrafish embryo 2hpf
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: 2 hr post fertilization
tissue: whole embryo
treated with: none (untreated)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol following manufactors specifications
|
| Label |
Cy5
|
| Label protocol |
Followed Agilent protocol substituting COT DNA with salmon sperm DNA at 10ug/ul
|
| |
|
| |
| Hybridization protocol |
Followed Agilent protocol
|
| Scan protocol |
Scanned on an Agilent High-Resolution C Scanner or an Axon 4000B.
Images were quantified using Agilent Feature Extraction Software.
|
| Description |
wt2_v_wt2
WT2h-expression
|
| Data processing |
Images were quantified and normalized (Lowess) using Agilent Feature Extraction Software v10.7.1.1. Active genes were filtered from maternal transcripts based on wt2_v_wt2 (control) experiment.
|
| |
|
| Submission date |
Jan 25, 2013 |
| Last update date |
Dec 09, 2013 |
| Contact name |
Steven Weicksel |
| E-mail(s) |
steven.weicksel@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
BMP
|
| Lab |
Sagerstrom
|
| Street address |
55 Lake Ave
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL14664 |
| Series (2) |
| GSE43756 |
Gene expression at four stages of the developing zebrafish embryo |
| GSE43758 |
Identification of nucleosome positions at hox TSSs during zebrafish early embryonic development |
|
