|
| Status |
Public on Jan 13, 2014 |
| Title |
PGC-1a-/- rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Cardiac biventricle of vehicle injected PGC-1a-/-bf/f/MerCre mouse (control)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cardiac biventricle
genotype/variation: PGC-1a KO (PGC-1b expression is unaffected)
|
| Treatment protocol |
At 2 months of age, mice were injected on two sequential days with 50mg/kg/day of Tamoxifen (10mg/ml) dissolved in sunflower seed oil to induce the Cre recombinase. Vehicle injected control mice were injected with sunflower seed oil alone at the same timepoints.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAzol B -followed manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
sscDNA were prepared from 450ng of total RNA using the Ambion’s WT expression kit protocol, followed by fragmentation and labeling using the WT Terminal label kit according to the protocol provided by affymetrix. The Affymetrix proprietary DNA labeling reagent covalently linked to biotin was used to label the sscDNA
|
| |
|
| Hybridization protocol |
Following the labeling of sscDNA, 2.5 microgram of sscDNA was used to prepare hybridization cocktail. The cocktail was immediately added to the Affymetrix WT GENE 1.0 ST chip and hybridized using GeneChip Hybridization oven 645 for 17hrs at 45C.
|
| Scan protocol |
The arrays were washed and stained using Fluidics protocol FS450_0007 on GeneChip Fluidics Station 450. Following wash and stain the GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
3 months of age (collected 1 m after control vehicle injection)
|
| Data processing |
The data was analyzed using RMA algorithm using the Expression Console software Version 1.1 default settings.
|
| |
|
| Submission date |
Jan 28, 2013 |
| Last update date |
Jan 13, 2014 |
| Contact name |
Ola Martin |
| E-mail(s) |
omartin@burnham.org
|
| Phone |
407-745-2000
|
| Organization name |
Sanford-Burnham Medical Research Institute
|
| Lab |
Dr. Dan Kelly
|
| Street address |
6400 Sanger Rd
|
| City |
Orlando |
| State/province |
FL |
| ZIP/Postal code |
32827 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE43798 |
Microarray of cardiac biventricle from PGC-1a-/-bf/f/MerCre mice |
|
