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Status
Public on Jun 15, 2006
Title
NIH3T3_4h_FGF_exp1
Sample type
RNA
Source name
NIH3T3, FGF 4 h
Organism
Mus musculus Characteristics
NIH3T3
Extracted molecule
total RNA
Extraction protocol
RNeasy total RNA extraction protocol (Qiagen)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
NIH3T3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% calf serum.To obtain quiescent cells, cells were washed with serum-free DMEM followed by culture for 48 h in DMEM containing 0.1% serum. Serum-starved NIH3T3 cells were stimulated with bFGF (50 ng/ml) for 4 h.
Data processing
MAS 5.0, scaled to 500
Submission date
Apr 28, 2006
Last update date
Aug 28, 2018
Contact name
Eisuke Nishida
E-mail(s)
nishida@lif.kyoto-u.ac.jp Phone
+81-75-753-4230
Organization name
Graduate School of Biostudies, Kyoto University
Department
Department of Cell and Developmental Biology
Street address
Kitashirakawa, Sakyo-ku
City
Kyoto
ZIP/Postal code
606-8502
Country
Japan
Platform ID
GPL1261 Series (1)
GSE4739 Temporal control of gene expression by ERK MAP kinase during cell cycle progression from G0/G1 to S phase
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
726.5
P
0.000297
AFFX-BioB-M_at
1135.7
P
0.000044
AFFX-BioB-3_at
769.3
P
0.00011
AFFX-BioC-5_at
1508.2
P
0.000044
AFFX-BioC-3_at
1650.7
P
0.000044
AFFX-BioDn-5_at
1976.4
P
0.000044
AFFX-BioDn-3_at
6632.2
P
0.000044
AFFX-CreX-5_at
16171.2
P
0.000044
AFFX-CreX-3_at
22776
P
0.000044
AFFX-DapX-5_at
12.2
A
0.659339
AFFX-DapX-M_at
4.6
A
0.814869
AFFX-DapX-3_at
33.8
A
0.147939
AFFX-LysX-5_at
13
A
0.396902
AFFX-LysX-M_at
21.3
A
0.51489
AFFX-LysX-3_at
21.1
P
0.036569
AFFX-PheX-5_at
2.8
A
0.945787
AFFX-PheX-M_at
3.7
A
0.843268
AFFX-PheX-3_at
29.9
A
0.340661
AFFX-ThrX-5_at
4
A
0.941556
AFFX-ThrX-M_at
4.1
A
0.712257
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