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Status
Public on Jun 15, 2006
Title
NIH3T3_7h_FGF_exp1
Sample type
RNA
Source name
NIH3T3, FGF 7 h
Organism
Mus musculus
Characteristics
NIH3T3
Extracted molecule
total RNA
Extraction protocol
RNeasy total RNA extraction protocol (Qiagen)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
NIH3T3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% calf serum.To obtain quiescent cells, cells were washed with serum-free DMEM followed by culture for 48 h in DMEM containing 0.1% serum. Serum-starved NIH3T3 cells were stimulated with bFGF (50 ng/ml) for 7 h.
Data processing
MAS 5.0, scaled to 500
Submission date
Apr 28, 2006
Last update date
Aug 28, 2018
Contact name
Eisuke Nishida
E-mail(s)
nishida@lif.kyoto-u.ac.jp
Phone
+81-75-753-4230
Organization name
Graduate School of Biostudies, Kyoto University
Department
Department of Cell and Developmental Biology
Street address
Kitashirakawa, Sakyo-ku
City
Kyoto
ZIP/Postal code
606-8502
Country
Japan
Platform ID
GPL1261
Series (1)
GSE4739
Temporal control of gene expression by ERK MAP kinase during cell cycle progression from G0/G1 to S phase
Relations
Reanalyzed by
GSE119085
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
896.3
P
0.00039
AFFX-BioB-M_at
1702.7
P
0.000044
AFFX-BioB-3_at
1230.1
P
0.000044
AFFX-BioC-5_at
2035.3
P
0.000044
AFFX-BioC-3_at
2018.4
P
0.000044
AFFX-BioDn-5_at
2783.2
P
0.000044
AFFX-BioDn-3_at
8489.3
P
0.000044
AFFX-CreX-5_at
21153.1
P
0.000044
AFFX-CreX-3_at
26934.2
P
0.000044
AFFX-DapX-5_at
3.2
A
0.617421
AFFX-DapX-M_at
5.9
A
0.876448
AFFX-DapX-3_at
12.1
A
0.794288
AFFX-LysX-5_at
17.6
A
0.250796
AFFX-LysX-M_at
19.7
A
0.440646
AFFX-LysX-3_at
20
A
0.262827
AFFX-PheX-5_at
2.9
A
0.86866
AFFX-PheX-M_at
5
A
0.883887
AFFX-PheX-3_at
38.9
A
0.175328
AFFX-ThrX-5_at
18.7
A
0.455413
AFFX-ThrX-M_at
23.7
A
0.165861
Total number of rows: 45101 Table truncated, full table size 1209 Kbytes .Supplementary file
Size
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File type/resource
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CEL