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Status
Public on Jun 15, 2006
Title
NIH3T3_7h_FGF_U0126_exp1
Sample type
RNA
Source name
NIH3T3, FGF 7 h + U0126 at - 0.5 h
Organism
Mus musculus
Characteristics
NIH3T3
Extracted molecule
total RNA
Extraction protocol
RNeasy total RNA extraction protocol (Qiagen)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
NIH3T3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% calf serum.To obtain quiescent cells, cells were washed with serum-free DMEM followed by culture for 48 h in DMEM containing 0.1% serum. Serum-starved NIH3T3 cells were stimulated with bFGF (50 ng/ml) for 7 h. U0126 (20 uM) was added 30 min before stimulation.
Data processing
MAS 5.0, scaled to 500
Submission date
Apr 28, 2006
Last update date
Aug 28, 2018
Contact name
Eisuke Nishida
E-mail(s)
nishida@lif.kyoto-u.ac.jp
Phone
+81-75-753-4230
Organization name
Graduate School of Biostudies, Kyoto University
Department
Department of Cell and Developmental Biology
Street address
Kitashirakawa, Sakyo-ku
City
Kyoto
ZIP/Postal code
606-8502
Country
Japan
Platform ID
GPL1261
Series (1)
GSE4739
Temporal control of gene expression by ERK MAP kinase during cell cycle progression from G0/G1 to S phase
Relations
Reanalyzed by
GSE119085
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
1236.2
P
0.000754
AFFX-BioB-M_at
1805.8
P
0.000044
AFFX-BioB-3_at
1463.6
P
0.000044
AFFX-BioC-5_at
2284.7
P
0.000044
AFFX-BioC-3_at
2233.9
P
0.000052
AFFX-BioDn-5_at
3101.4
P
0.000044
AFFX-BioDn-3_at
8966.3
P
0.000044
AFFX-CreX-5_at
22283.6
P
0.000052
AFFX-CreX-3_at
28782.6
P
0.000044
AFFX-DapX-5_at
9.2
A
0.396902
AFFX-DapX-M_at
3.9
A
0.737173
AFFX-DapX-3_at
17.2
A
0.455409
AFFX-LysX-5_at
13.3
A
0.514891
AFFX-LysX-M_at
15.1
A
0.382579
AFFX-LysX-3_at
6.6
A
0.287743
AFFX-PheX-5_at
3.8
A
0.814869
AFFX-PheX-M_at
4.5
A
0.783476
AFFX-PheX-3_at
11.9
A
0.382599
AFFX-ThrX-5_at
5.4
A
0.712257
AFFX-ThrX-M_at
17.5
A
0.216524
Total number of rows: 45101 Table truncated, full table size 1208 Kbytes .Supplementary file
Size
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File type/resource
GSM107402.CEL.gz
5.5 Mb
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CEL