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Status
Public on Jun 15, 2006
Title
NIH3T3_12h_FGF_U0126_exp1
Sample type
RNA
Source name
NIH3T3, FGF 12 h + U0126 at - 0.5 h
Organism
Mus musculus Characteristics
NIH3T3
Extracted molecule
total RNA
Extraction protocol
RNeasy total RNA extraction protocol (Qiagen)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
NIH3T3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% calf serum.To obtain quiescent cells, cells were washed with serum-free DMEM followed by culture for 48 h in DMEM containing 0.1% serum. Serum-starved NIH3T3 cells were stimulated with bFGF (50 ng/ml) for 12 h. U0126 (20 uM) was added 30 min before stimulation.
Data processing
MAS 5.0, scaled to 500
Submission date
Apr 28, 2006
Last update date
Aug 28, 2018
Contact name
Eisuke Nishida
E-mail(s)
nishida@lif.kyoto-u.ac.jp Phone
+81-75-753-4230
Organization name
Graduate School of Biostudies, Kyoto University
Department
Department of Cell and Developmental Biology
Street address
Kitashirakawa, Sakyo-ku
City
Kyoto
ZIP/Postal code
606-8502
Country
Japan
Platform ID
GPL1261 Series (1)
GSE4739 Temporal control of gene expression by ERK MAP kinase during cell cycle progression from G0/G1 to S phase
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
695.1
P
0.000297
AFFX-BioB-M_at
909.2
P
0.000044
AFFX-BioB-3_at
656
P
0.00007
AFFX-BioC-5_at
1242.6
P
0.00007
AFFX-BioC-3_at
1550.4
P
0.000044
AFFX-BioDn-5_at
1752.9
P
0.000044
AFFX-BioDn-3_at
6647.6
P
0.000044
AFFX-CreX-5_at
14309.5
P
0.000052
AFFX-CreX-3_at
22431.3
P
0.000044
AFFX-DapX-5_at
6.5
A
0.737173
AFFX-DapX-M_at
12.5
A
0.631562
AFFX-DapX-3_at
22.1
A
0.440641
AFFX-LysX-5_at
4.3
A
0.794268
AFFX-LysX-M_at
30.8
A
0.262827
AFFX-LysX-3_at
23.7
A
0.239044
AFFX-PheX-5_at
1.3
A
0.973889
AFFX-PheX-M_at
2.7
A
0.814869
AFFX-PheX-3_at
46.6
A
0.205732
AFFX-ThrX-5_at
3.6
A
0.84329
AFFX-ThrX-M_at
11.7
A
0.514891
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