|
| Status |
Public on Jun 30, 2013 |
| Title |
DDC-expressing 3T3 cells in L-Lysine Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
3T3 Fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 3T3 Fibroblasts
growth protocol: L-Lysine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA extracted with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser and Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX mouse-ref8 v2 R3 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
LLys_rep3
replicate 3
|
| Data processing |
The data were median centered using R
|
| |
|
| Submission date |
Jan 30, 2013 |
| Last update date |
Jun 30, 2013 |
| Contact name |
Nicholas Gauthier |
| Organization name |
MSKCC
|
| Street address |
1275 York Avenue, Box #460
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE43894 |
Expression analysis of 3T3 cells that express Arabidopsis-derived Diaminopimelate Decarboxylase (DDC) in response to growth on meso-2,6-diaminopimelic acid (DAP) versus L-Lysine |
|
