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Sample GSM1074214 Query DataSets for GSM1074214
Status Public on Jan 31, 2013
Title H275R_HOMO_rep1
Sample type RNA
 
Source name E12.5 whole embryo
Organism Mus musculus
Characteristics developmental stage: embryonic day E12.5
tissue: whole embryo
genotype: Klf3 H275R/H275R
Treatment protocol RNA was extracted from E12.5 embryos resulting from timed matings
Growth protocol All mice were housed and maintained in standard facilities in accordance with the guidelines of the Canadian Council of Animal Care.
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using TRI-Reagent, according to manufacturer’s guidelines (Sigma-Aldrich). RNA samples were further purified using RNeasy columns (Qiagen)
Label biotin
Label protocol RNA was labeled using the Affymetrix IVT Express kit
 
Hybridization protocol RNA was hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
Scan protocol Arrays were scanned following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
Data processing Microarray data were analyzed using Partek Genomic Suite v6.6 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
 
Submission date Jan 30, 2013
Last update date Jan 31, 2013
Contact name Alister Funnell
E-mail(s) a.funnell@unsw.edu.au
Organization name University of NSW
Street address School of Biotechnology and Biomolecular Sciences
City University of NSW
ZIP/Postal code 2052
Country Australia
 
Platform ID GPL16570
Series (1)
GSE43908 Microarray analysis of wildtype, Klf3 H275R homozygous and heterozygous, Klf3 CH gene trap homozygous and heterozygous E12.5 embryos

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
17200001 7.9834
17200003 8.10596
17200005 5.9194
17200007 6.13412
17200009 7.71976
17200011 6.67499
17200013 6.43213
17200015 6.91307
17200017 4.7683
17200019 5.9089
17200021 6.35841
17200023 8.54975
17200025 7.60517
17200027 7.08168
17200029 5.44797
17200031 5.25003
17200033 5.11933
17200035 5.69705
17200037 6.50956
17200039 5.53517

Total number of rows: 39137

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1074214_H275R_HOMO_01.CEL.gz 9.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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