|
Status |
Public on Jul 09, 2013 |
Title |
B4-159 |
Sample type |
RNA |
|
|
Source name |
Medulloblastoma, Ptch+/- model
|
Organism |
Mus musculus |
Characteristics |
strain: CBA/J genotype: Ptch+/-;T2Onc+/- age(weeks): 10 organ: Brain, Cerebellum disease state: Tumour insert in network gene: n/a insert in nfia: n/a
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Qiagen RNeasy Lipid Tissue mini kit (Qiagen, Hilden, Germany) according to manufacturer's instructions.Quality control was performed using an Agilent 2100 Bioanalyser.
|
Label |
biotin
|
Label protocol |
Approximately 200ng of each RNA was amplified and Biotinylated using the Illumina TotalPrep RNA amplification kit (Applied Biosystems, Foster City, CA. USA), with the size distribution of cRNA being assessed using the Agilent Bioanalyser.
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|
|
Hybridization protocol |
Approximately 750ng of each cRNA was hybridised using the standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Control Group 2 - Tumour which developed in Ptch+/- predisposition background with no transposition
|
Data processing |
Standard Illumina processing followed by RMA quantile normalisation.
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|
|
Submission date |
Feb 01, 2013 |
Last update date |
Jul 09, 2013 |
Contact name |
Michael Stewart Jackson |
E-mail(s) |
m.s.jackson@ncl.ac.uk
|
Phone |
+44 191 241 8677
|
Organization name |
Newcastle University
|
Department |
Institute of Genetic Medicine
|
Street address |
Central Parkway
|
City |
Newcastle upon Tyne |
ZIP/Postal code |
NE1 3BZ |
Country |
United Kingdom |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE43994 |
Identification of genes involved in SHH subgroup medulloblastoma using murine whole-body Sleeping Beauty insertional mutagenesis |
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