|
| Status |
Public on Feb 19, 2014 |
| Title |
GIF-14_untreated_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
GIF-14_untreated
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Fetal gastric epithelial cells
genotype: Runx3-/-;p53-/- genotype
|
| Treatment protocol |
Recombinant TGF-β1 for 24 h at a final concentration of 2.5 ng/ml
|
| Growth protocol |
Dulbecco’s Modified Eagle Medium with 4500 mg/l glucose supplemented with 10% FBS, 100 units/ml penicillin and 100 µg/ml streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy Mini Kit according to manufacturer's protocol. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with Ambion TotalPrep RNA Amplification Kit for Illumina
|
| |
|
| Hybridization protocol |
Incubated at 58oC for 16 hours in Illumina Hyb Chamber with rocker speed at 5
|
| Scan protocol |
Standard Illumina scanning protocol using Scan Factor of 3
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile and average normalisation with Illumina GenomeStudio
|
| |
|
| Submission date |
Feb 04, 2013 |
| Last update date |
Feb 19, 2014 |
| Contact name |
Tuan Zea Tan |
| E-mail(s) |
csittz@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Street address |
14 Medical Drive, #11-01, Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE44055 |
EMT-induced stemness and tumorigenicity are fueled by an oncogenic EGF/Ras pathway |
|
